The role of electroactive biofilms in enhanced para-chlorophenol transformation collaborated with biosynthetic...

Bioremediation is a cost-effective strategy for decomposition of chlorinated organic contaminants, but its application is often hindered by the generation of toxic chlorinated byproducts. Though the design of functional biofilms, incorporating microbially-inspired catalytic materials, has emerged as a promising solution for tackling the byproducts issues, the microbial mechanisms driving these processes remain inadequately understood. This study demonstrates a hybrid electroactive biofilm (EAB)-palladium nanoparticles (Pd NPs) system that effectively separates the dechlorination and mineralization of para-chlorophenol (4-CP), and most importantly, it provides new insights into the microbial and genetic roles of EABs in this process. Under an applied potential of -0.6 V, Pd NPs via palladate reduction were biogenically synthesized and deposited on the cytomembranes within the biofilm, achieving an 82 % decrease in 4-CP concentration within 48 h. The ultra-performance liquid chromatogram and mass spectrum confirmed that 4-CP was initially dechlorinated to phenol by the biogenic Pd NPs before undergoing further degradation by the biofilm, effectively preventing toxic chlorinated byproducts. The Dechloromonas, Pseudomonas, and Geobacter were identified as predominant genera in the system and the metagenomics analysis noted increased relative abundance of ring-cleavage genes like pcaG, dmpB/xylE, and catA. Importantly, the abundance of dmpB/xylE was primarily associated with Dechloromonas and Pseudomonas, further highlighted that the dmpB/xylE-pathway was important for rapid 4-CP decomposition in the system. This study advances the understanding of EAB-Pd NPs synergy, showcasing an innovative and sustainable approach for the efficient removal of halogenated pollutants.