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Updated: May 23, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Biocatalysis: Important considerations for testing and evaluation of biocatalysts
Dirk Tischler1, Giovanni Davide Barone2, Jose Munoz-Munoz3
1Microbial Biotechnology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Bochum, Germany.
Abstract:
Selecting the appropriate mode of biocatalysis application is crucial for optimizing efficiency and sustainability. This chapter provides a comprehensive guide on key metrics to describe biocatalyst performance, including kinetic parameters such as reaction rates, cofactor requirements, dissociation constants (KD), maximum velocities (Vmax), turnover numbers (kcat), and Michaelis constants (KM). Additionally, it discusses biocatalysis metrics like turnover frequency (TOF), environmental factors (E-Factor), atom economy, productivities, and Life Cycle Assessment (LCA). The chapter also explores application types, focusing on whole-cell and cell-free enzyme applications, and offers a practical guide on selecting the most suitable mode of application based on specific project requirements. By integrating these considerations, researchers can effectively harness biocatalysis for innovative and sustainable solutions in various industrial processes.
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