Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioremediation00:46

Bioremediation

18.1K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycerol-mediated improvement of heterologous aurachin D production in E. coli.

Applied microbiology and biotechnology·2026
Same author

Reaction Engineering of In Vitro Natural Product Biosynthesis: Challenges and Strategies.

Chembiochem : a European journal of chemical biology·2025
Same author

Genome Reduction Improves Recombinant Benzoxazole Production in <i>Myxococcus xanthus</i>.

ACS synthetic biology·2025
Same author

Discovery and In Vitro Reconstitution of Closoxazole Biosynthesis from Pyxidicoccus fallax.

Chembiochem : a European journal of chemical biology·2025
Same author

Aurachins, Bacterial Antibiotics Interfering with Electron Transport Processes.

Antibiotics (Basel, Switzerland)·2023
Same author

Intermediates and shunt products of massiliachelin biosynthesis in <i>Massilia</i> sp. NR 4-1.

Beilstein journal of organic chemistry·2023

Related Experiment Video

Updated: May 10, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.2K

Whole-cell biocatalysis with Myxococcus xanthus.

Lea Winand1, Markus Nett1

  • 1TU Dortmund University, Department of Biochemical and Chemical Engineering, Dortmund, Germany.

Methods in Enzymology
|April 27, 2025
PubMed
Summary

Whole-cell biocatalysis using the bacterium Myxococcus xanthus offers a cost-effective alternative to chemical synthesis. This method leverages M. xanthus

Keywords:
BiocatalysisBiotransformationHeterologous expressionMyxococcus xanthusNatural products

More Related Videos

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
11:45

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Published on: June 20, 2018

9.4K
Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
10:59

Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

Published on: August 6, 2010

12.2K

Related Experiment Videos

Last Updated: May 10, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.2K
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
11:45

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Published on: June 20, 2018

9.4K
Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
10:59

Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography

Published on: August 6, 2010

12.2K

Area of Science:

  • Biocatalysis and organic synthesis
  • Microbial biotechnology and metabolic engineering

Background:

  • Biocatalysis is a preferred method in organic synthesis due to its high selectivity and reduced by-product formation compared to chemical catalysis.
  • Whole-cell biocatalysts are favored over isolated enzymes due to cost-effectiveness, despite challenges with cofactor dependency and enzyme isolation.
  • Myxococcus xanthus is a promising microbial host for producing natural products, including antibiotics, owing to its xenobiotic tolerance and metabolic versatility.

Purpose of the Study:

  • To provide detailed procedures for implementing whole-cell biocatalysis using Myxococcus xanthus.
  • To facilitate the use of M. xanthus as a cell factory for producing valuable compounds.
  • To enable the engineering of M. xanthus for enhanced biosynthesis of natural products.

Main Methods:

  • Description of whole-cell biocatalysis protocols tailored for M. xanthus.
  • Methodologies for constructing expression plasmids compatible with M. xanthus.
  • Procedures for the efficient transfer of expression constructs into M. xanthus.

Main Results:

  • Established protocols for effective whole-cell biocatalysis in M. xanthus.
  • Demonstrated feasibility of using M. xanthus as a versatile host for biocatalytic applications.
  • Provided a foundation for further metabolic engineering of M. xanthus for natural product synthesis.

Conclusions:

  • Myxococcus xanthus is a robust and versatile platform for whole-cell biocatalysis, particularly for natural product biosynthesis.
  • The described methods enable the utilization and genetic manipulation of M. xanthus for pharmaceutical and fine chemical production.
  • This work supports the broader application of microbial biocatalysis in sustainable chemical manufacturing.