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Updated: May 10, 2025
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Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
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The Nucleoporin CPR5 Modulates Plant Immunity via Guanylate-Binding Proteins
Leiwen Pan1, Shun Peng1,2, Yuehui Zhang1
1Shanghai Engineering Research Center of Plant Germplasm Resources, Shanghai Collaborative Innovation Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Molecular Plant Pathology
|April 27, 2025
Summary
A novel mutation in GBPL2 suppresses defects in plant immunity related to CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5). This suppressor mutation reveals that GBPL2 interacts with GBPL3 to regulate immune signaling downstream of CPR5.
Area of Science:
- Plant molecular biology
- Plant immunity and defense mechanisms
- Cellular transport and signaling
Background:
- Nuclear pore complex is crucial for immune signal transport.
- CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5) and GUANYLATE-BINDING PROTEIN-LIKE 3 (GBPL3) are involved in plant immunity.
- The function of GBPL2 within the GUANYLATE-BINDING PROTEIN-LIKE (GBPL) family was previously unknown.
Purpose of the Study:
- To investigate the unknown role of GBPL2 in plant immunity.
- To identify genetic factors that modulate the CPR5 immune pathway.
- To elucidate the molecular mechanism of GBPL family proteins in plant defense.
Main Methods:
- Genetic screening to identify suppressors of the cpr5 mutant.
- Characterization of a gain-of-function mutation in GBPL2 (scpr23).
- Analysis of protein localization, epistatic interactions, and gene expression.
Main Results:
- A gain-of-function mutation in GBPL2 (scpr23) was identified, which fully suppresses the cpr5 mutant phenotype.
- The scpr23 mutation alters GBPL2 localization from the ER to the nuclear envelope by affecting a nuclear export signal (NES).
- Epistatic analysis confirmed that GBPL2's function in suppressing cpr5 is mediated through GBPL3, suggesting a GBPL2-GBPL3 signaling complex downstream of CPR5.
Conclusions:
- GBPL2, particularly through its scpr23 mutant form, plays a significant role in regulating plant immunity.
- The GBPL family proteins, including GBPL2 and GBPL3, form a signaling complex downstream of CPR5.
- GBPL2 acts as a plant ortholog to mammalian ATLASTIN-1, highlighting conserved functions in membrane dynamics and immunity.
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