Preventive Effects of Vanillic Acid Against Lung Inflammation and Oxidative Stress Induced by Dust Particles in...

To evaluate dose-dependent cytotoxicity effects of indoor dust particles (DPs) collected from Neyshabur, Iran, in vitro on A545 cells and in vivo on lungs of healthy male Wistar rats, as well as the antioxidant effects of vanillic acid (VA) against DP inhalation. Heavy metal levels in DPs collected from high-traffic (HT), medium-traffic, low-traffic or rural (LT) zones were measured, and their cytotoxicity effects were evaluated by MTT assay. In vivo evaluations were conducted after rats were exposed to DPs collected from HT or LT in the presence or absence of VA. Exposure to DPs increased the activity of serum superoxide dismutase; the serum level of malondialdehyde; and mRNA expression of TNFα, IL6, CXCL15 and CYP1A1 in the lung homogenate groups receiving HT and LT compared to the control group. DP effects in the groups receiving HT were higher than those of LT. Concomitant VA intake attenuated the adverse effects mediated by DPs in the HT and LT groups. DPs had adverse effects on the lungs of healthy rats, probably because of the accumulated oxidative stress agents. VA could ameliorate the effects of DPs and may be considered as a protective substance against the undesirable effects of DPs.