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ROPISA Strategy for In-Situ Loading in Polypeptide Nanoparticles.

Rahul Nisal1, Parshuram Kambale1, Shahidkhan Pathan1

  • 1Department of Chemistry, Indian Institute of Science Education and Research (IISER), Dr. Homi Bhabha Road, Pune, Maharashtra, 411008, India.

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Summary

We developed a new method for creating fluorescent polypeptide nanoparticles using ring-opening polymerization induced self-assembly (ROPISA). These biocompatible nano-assemblies are effective for cellular bioimaging applications.

Keywords:
Imaging‐agentsPeptidesRing‐opening polymerizationSelf‐assemblyTemplate synthesis

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Nanotechnology

Background:

  • Developing novel polypeptide nano-assemblies is crucial for advanced bioimaging.
  • In-situ encapsulation methods are needed for efficient loading of fluorescent dyes.

Purpose of the Study:

  • To develop a ring-opening polymerization induced self-assembly (ROPISA) strategy for in-situ encapsulation of fluorescent dyes in poly(ʟ-serine) based polypeptide nano-assemblies.
  • To demonstrate the cellular bioimaging application of these fluorescent polypeptide nano-assemblies.

Main Methods:

  • Tailor-made bulky ʟ-serine N-carboxyanhydride monomer polymerization using PEG-amine macroinitiator in water.
  • In-situ encapsulation of water-soluble (Rhodamine B, HPTS) and water-insoluble (Nile red) fluorescent dyes.
  • Characterization of nanoparticle size, morphology, stability, and biocompatibility.

Main Results:

  • Stable polypeptide block copolymer dispersions with spherical nanoparticle morphology (25 nm) were obtained.
  • Successful in-situ encapsulation of various fluorescent dyes was achieved.
  • The polypeptide nanoparticles demonstrated non-toxicity to mammalian cells and non-hemolytic properties.
  • Efficient cellular uptake and cytosolic internalization were confirmed via confocal microscopy.

Conclusions:

  • The ROPISA method provides a versatile platform for creating fluorescent polypeptide nano-assemblies.
  • These nano-assemblies are suitable for direct application in biological systems, particularly for cellular bioimaging.
  • This approach opens new avenues for polypeptide nano-formulations in material and biomedical fields.