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Updated: May 10, 2025

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
Published on: September 2, 2019
Agrin/Dok-7-induced JPH2 phosphorylation in muscle cells is involved in AChR clustering
Beibei Wang1,2,3, Mengge Cui2,3,4, Huan Liu2,3,5
1Department of Pharmacology, School of Medicine, Affiliated Hospital, Hebei University of Engineering, Handan, China.
Abstract:
The neuromuscular junction (NMJ) performs the crucial function of controlling skeletal muscle contraction. NMJ formation depends on the Agrin/Lrp4/MuSK/Dok-7 signaling pathway. However, signaling downstream of Dok-7 remains incompletely understood. Here we used the phosphorylated iTRAQ technique to identify downstream molecules of Dok-7 in muscle cells. We found 16 Agrin/Dok-7-mediated serine/threonine phosphorylated proteins, and we validated the role of one phosphorylated protein, JPH2, in regulating AChR clustering. Our phosphoproteomics analysis sheds light on the underappreciated signaling network downstream of Agrin/Dok-7, thus providing new clues for understanding pathogenesis and developing treatment methods for neuromuscular diseases.
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