Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intracellular processing of prolactin.

W S Oetting, A M Walker

    Endocrinology
    |October 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers investigated prolactin (PRL) variants using two-dimensional gel electrophoresis. They found that PRL is processed into smaller, secreted derivatives, suggesting these may be biologically significant relatives.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adolescent ethanol exposure reprograms HMGB1 responsivity to adult ethanol challenge in males.

    Physiology & behavior·2026
    Same author

    Computed tomography versus radiography for assessment of canine humeral condylar fracture malreductions.

    New Zealand veterinary journal·2025
    Same author

    Development of a novel Bluetooth Low Energy device for proximity and location monitoring in grazing sheep.

    Animal : an international journal of animal bioscience·2024
    Same author

    Neuroprotection by anaesthetics in rodent models of traumatic brain injury: a systematic review and network meta-analysis.

    British journal of anaesthesia·2018
    Same author

    Genome-wide association study identifies the common variants in CYP3A4 and CYP3A5 responsible for variation in tacrolimus trough concentration in Caucasian kidney transplant recipients.

    The pharmacogenomics journal·2017
    Same author

    Point-of-care paediatric gastric sonography: can antral cut-off values be used to diagnose an empty stomach?

    British journal of anaesthesia·2017
    Same journal

    Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

    Endocrinology·2026
    Same journal

    Long Non-Coding RNAs Mediate Endocrine Signaling and Resistance in Prostate Cancer.

    Endocrinology·2026
    Same journal

    Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

    Endocrinology·2026
    Same journal

    Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

    Endocrinology·2026
    Same journal

    Discovery proteomics identification of factors contributing to gonadotropin β expression.

    Endocrinology·2026
    Same journal

    3D genome architecture and epigenetic regulation of lineage identity in advanced prostate cancer.

    Endocrinology·2026
    See all related articles

    Area of Science:

    • Endocrinology and Molecular Biology
    • Protein Biochemistry and Cell Signaling

    Background:

    • Prolactin (PRL) is known to exist in various molecular weight and charge variants.
    • The precise relationships between these PRL variants, particularly lower molecular weight forms, remain incompletely understood.

    Purpose of the Study:

    • To elucidate the relationships among charge and lower molecular weight variants of prolactin (PRL).
    • To investigate the processing and potential biological significance of PRL derivatives.

    Main Methods:

    • Two-dimensional gel electrophoresis was employed on pituitary and pituitary cell extracts.
    • Silver staining and autoradiography were used to visualize PRL isoforms and processing products.
    • Western blotting confirmed the immunological relationship of arc products to PRL.

    Related Experiment Videos

    Main Results:

    • Three major charge isoforms of 24,000 mol wt PRL were identified, along with an arc of smaller, more negatively charged products.
    • These arc products were immunologically related to PRL and were detectable in both cell extracts and culture medium.
    • Inhibition of pre-PRL to PRL processing resulted in doublet spots within the arc, indicating intracellular processing.

    Conclusions:

    • Prolactin (PRL) undergoes intracellular processing into multiple smaller derivatives.
    • These PRL derivatives are secreted and may represent biologically important relatives of the parent hormone.