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Monitoring Protein Stability In Vivo Using an Intein-Based Biosensor.

John S Smetana1, Tia M Ariagno1, Ahyun Son2

  • 1Deptartment of Biological Sciences, Murray State University, Murray, KY, USA.

Bio-Protocol
|April 28, 2025
PubMed
Summary
This summary is machine-generated.

Inteins are protein splicing elements used to engineer biosensors. This study presents a novel intein-based biosensor for measuring protein stability in vivo, linking it to antibiotic resistance and fluorescence.

Keywords:
BiosensorChaperoneInteinProtein foldingProtein splicingProtein stability

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Inteins are protein sequences that catalyze their own excision from a precursor protein.
  • Inteins facilitate protein splicing, a unique reaction that rearranges peptide bonds.
  • Intein-mediated protein splicing has been harnessed for various protein engineering applications.

Purpose of the Study:

  • To develop a novel intein-based biosensor for measuring protein stability in vivo.
  • To create a dual-readout biosensor combining antibiotic resistance and fluorescence.
  • To assess the impact of chaperonins on protein stability using the developed biosensor.

Main Methods:

  • Constructed intein-protein fusions where excision confers antibiotic resistance.
  • Utilized logarithmic dilution and antibiotic agar plates to generate quantitative survival curves.
  • Developed a dual biosensor incorporating fluorescence as a secondary readout.
  • Investigated the effect of co-expressing GroEL on biosensor performance.

Main Results:

  • The biosensor detected a 100,000-fold difference in survival between stable and unstable proteins.
  • The dual biosensor quantitatively correlated protein stability with both antibiotic resistance and fluorescence.
  • Co-expression of GroEL enhanced both survival and fluorescence, indicating improved protein stability.

Conclusions:

  • A novel intein-based biosensor enables in vivo measurement of protein stability.
  • The biosensor offers a sensitive and quantitative method for assessing protein folding and stability.
  • This tool expands the capabilities for studying protein homeostasis within cellular environments.