Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

37.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
37.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive whole genome sequencing-based pharmacogenomics profiling using a personalized genome interpretation workflow.

Frontiers in pharmacology·2026
Same author

Development of a New Portable Genetic Analyzer for Point-of-Care Molecular Genetics and Pharmacogenomics Analysis.

Human mutation·2026
Same author

Association of <i>ABCB1</i> and <i>CYP2C19</i> polymorphisms with major adverse cardiovascular complications in patients taking clopidogrel.

Frontiers in cardiovascular medicine·2026
Same author

Thwarting the biothreat in the 21st century: means and methods.

Future microbiology·2026
Same author

Cardiologists' perspectives on pharmacogenomics implementation in a hybrid health system: A qualitative study from the United Arab Emirates.

PloS one·2026
Same author

STRIPE partners in precision medicine: international perspective.

The pharmacogenomics journal·2026

Related Experiment Video

Updated: May 10, 2025

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.2K

Scalable Alkaline Extraction Protocol for Microbial DNA Screening by PCR.

Stavroula Goudoudaki1, Manousos E Kambouris1,2, Stavroula Kritikou3

  • 1Plant Protection Division of Patras (PPDP), ELGO-Demeter, Patras, Greece.

Bio-Protocol
|April 28, 2025
PubMed
Summary

This study presents a cost-effective NaOH-based DNA extraction method for rapid nucleic acid amplification tests (NAAT). It offers a robust solution for molecular diagnostics in resource-limited settings and crisis situations.

Keywords:
Alkaline extractionBiosurveillanceDNA extraction kitsEcologyMinimal resource analysisOne HealthResource-limited settingsalcohol precipitation

More Related Videos

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

26.1K
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.5K

Related Experiment Videos

Last Updated: May 10, 2025

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.2K
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

26.1K
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.5K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Commercial DNA extraction kits are often proprietary and sample-specific, hindering supply chain flexibility during crises.
  • Existing rapid DNA extraction protocols, while fast, may not be robust enough for diverse sample types or resource-limited settings.
  • Supply chain disruptions and surges in demand necessitate alternative, adaptable DNA extraction methods for molecular diagnostics.

Purpose of the Study:

  • To develop and validate a simplified, cost-effective DNA extraction protocol using sodium hydroxide (NaOH).
  • To enable rapid DNA extraction for nucleic acid amplification tests (NAAT), including polymerase chain reaction (PCR), in various settings.
  • To provide a method that is adaptable for both immediate use (crude extract) and for storage/transport (refined extract).

Main Methods:

  • Biological samples are treated with 0.2 M NaOH, incubated at 75°C, and then neutralized with Tris buffer for crude extract preparation.
  • Crude extract is serially diluted and used directly as a template for PCR, bypassing purification and quantification.
  • A refined extract is produced by precipitating nucleic acids with isopropanol after HCl treatment, allowing for storage and transport.

Main Results:

  • The NaOH-based method effectively lyses cells and nuclei, yielding DNA suitable for PCR amplification.
  • Both crude and refined extracts demonstrated utility in PCR, with the refined extract offering improved amplification quality and sensitivity.
  • The protocol significantly reduces costs, environmental impact, and logistical requirements compared to traditional kits.

Conclusions:

  • This NaOH-based DNA extraction method offers a sustainable and adaptable solution for molecular diagnostics, particularly in resource-limited settings and emergency situations.
  • The dual-version approach (crude for immediate use, refined for storage/transport) enhances flexibility and throughput during supply chain disruptions.
  • The method's simplicity, low cost, and minimal footprint make it a valuable alternative for increasing testing capacity and resilience.