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  1. Home
  3. Tricellulin Facilitates Colorectal Cancer Metastasis Through Activation Of The Tgfβ/smad2/3 Signalling Pathway.
  1. Home
  3. Tricellulin Facilitates Colorectal Cancer Metastasis Through Activation Of The Tgfβ/smad2/3 Signalling Pathway.

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Tricellulin facilitates colorectal cancer metastasis through activation of the TGFβ/SMAD2/3 signalling pathway.

Wenfang Yang1, Ruoxi Cheng1, Mengbin Qin1

  • 1Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Frontiers in Oncology
|April 28, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Tricellulin promotes colorectal cancer (CRC) metastasis by activating the TGF-β/SMAD2/3 pathway. This protein forms a positive feedback loop, enhancing tumor growth and suggesting new therapeutic targets for CRC.

Keywords:
TGFβ/Smad signalingcolorectal cancerepithelial-mesenchymal transitionmetastasistricellulin

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Area of Science:

  • Molecular biology
  • Oncology
  • Cell biology

Background:

  • Tricellulin, a tight junction protein, is implicated in cancer progression.
  • Its specific role in colorectal cancer (CRC) progression requires further elucidation.

Purpose of the Study:

  • To investigate the function and underlying mechanisms of tricellulin in CRC progression.
  • To determine the relationship between tricellulin expression and patient prognosis.

Main Methods:

  • Western blot, RT-qPCR, and immunohistochemistry were used to assess protein expression.
  • In vitro assays (cell proliferation, migration, invasion) and in vivo xenograft models evaluated tricellulin's biological functions.
  • The TGF-β1/SMAD2/3 pathway activation was analyzed.

Main Results:

  • Tricellulin was significantly upregulated in CRC tissues and correlated with poor prognosis.
  • Overexpression of tricellulin enhanced CRC cell proliferation, migration, and invasion in vitro.
  • Tricellulin activated the TGF-β1/SMAD2/3 pathway, promoting epithelial-mesenchymal transition (EMT) and tumor growth in vivo.

Conclusions:

  • Tricellulin promotes colorectal cancer metastasis via the TGF-β/SMAD2/3 pathway.
  • A positive feedback loop exists where tricellulin regulates TGF-β1, and vice versa, driving CRC growth and metastasis.
  • These findings offer novel insights into CRC progression and identify potential therapeutic targets.