Anticancer effect of Phyllanthus reticulatus methanolic leaf extract on HCT-116 colon cancer cell line using GC-MS and FTIR analysis
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View abstract on PubMed
Summary
This summary is machine-generated.Phyllanthus reticulatus leaf extract exhibits significant antioxidant and anticancer properties, showing 28.56% inhibition of colon cancer cells. This study reveals its rich phytochemical profile, supporting its traditional medicinal uses and potential for drug development.
Area Of Science
- Ethnobotany
- Phytochemistry
- Pharmacology
Background
- Phyllanthus reticulatus, a plant from the Euphorbiaceae family, is traditionally used for various medicinal purposes.
- Existing literature suggests hepatoprotective, hypolipidemic, analgesic, anti-inflammatory, antimalarial, cytotoxic, and antimicrobial properties.
- The plant's leaves are particularly noted for potential applications in managing diabetes and diarrhea.
Purpose Of The Study
- To investigate the phytochemical composition of Phyllanthus reticulatus methanolic leaf extract.
- To evaluate the antioxidant and anticancer properties of the extract.
- To establish the spectroscopic characteristics and anticancer effects on colon cancer cells.
Main Methods
- Phytochemical analysis and Gas Chromatography-Mass Spectrometry (GC-MS) to identify active components.
- Fourier-Transform Infrared (FTIR) spectroscopy to detect functional groups.
- DPPH and PM assays for antioxidant activity and in vitro testing against HCT-116 colon cancer cells.
Main Results
- GC-MS analysis identified nine major peaks with active chemical components.
- FTIR analysis confirmed the presence of six biologically active functional groups.
- The extract contained significant phenolic (93.44 mg/g) and flavonoid (55.35 mg/g) content.
- Antioxidant assays indicated significant free radical scavenging activity.
- The extract demonstrated anticancer properties, inhibiting HCT-116 colon cancer cell growth by 28.56% at 24 hours in a dose-dependent manner.
Conclusions
- This study provides the first report on the spectroscopic and anticancer properties of Phyllanthus reticulatus leaf extract against colon cancer.
- The findings support the traditional medicinal uses of the plant and highlight its potential as a source of anticancer compounds.
- Further research into the bioactive constituents and mechanisms of action is warranted for potential drug development.
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