Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human endogenous retrovirus envelope proteins alter extracellular vesicle cellular interactions and biodistribution.

bioRxiv : the preprint server for biology·2026
Same author

Updating MISEV: When, What, How, and Why?

Journal of extracellular vesicles·2026
Same author

Five Years of EVClub-From Journal Club to Worldwide Discussion Hub.

Journal of extracellular vesicles·2025
Same author

Extracellular Vesicles Favor Early Peripheral Immunosenescence Through Modulation of the Senescence-Associated Secretory Phenotype in HIV Infection.

Aging cell·2025
Same author

Magnetically Labelled iPSC-Derived Extracellular Vesicles Enable MRI/MPI-Guided Regenerative Therapy for Myocardial Infarction.

Journal of extracellular vesicles·2025
Same author

Tumoural Hypoxic Extracellular Vesicles Foster a Protective Microenvironment in Triple-Negative Breast Cancer.

Journal of extracellular biology·2025

Related Experiment Video

Updated: May 10, 2025

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.3K

Exploring the Adhesion Properties of Extracellular Vesicles for Functional Assays.

Bianca C Pachane1,2, Bess Carlson1, Suzanne E Queen1

  • 1Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine Baltimore Maryland USA.

Journal of Extracellular Biology
|April 28, 2025
PubMed
Summary

Extracellular vesicles (EVs) can be immobilized on standard borosilicate glass coverslips for imaging techniques. Poly-L-lysine enhances uniform distribution but isn

Keywords:
adhesionextracellular vesiclessuper‐resolution microscopy

More Related Videos

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.4K
Setting a Successful Sorting for Extracellular Vesicle Isolation
08:37

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

831

Related Experiment Videos

Last Updated: May 10, 2025

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.3K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.4K
Setting a Successful Sorting for Extracellular Vesicle Isolation
08:37

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

831

Area of Science:

  • Biotechnology
  • Materials Science
  • Microscopy

Background:

  • Extracellular vesicles (EVs) exhibit 'stickiness' that complicates processing but can be leveraged for surface immobilization.
  • Current methods for EV immobilization, such as molecular affinity, can be costly and limit the population examined.
  • Super-resolution microscopy (SRM) requires stable EV immobilization for detailed analysis.

Purpose of the Study:

  • To investigate the interaction of EVs with borosilicate glass and quartz coverslips for immobilization.
  • To evaluate the effect of poly-L-lysine (PLL) pre-coating on EV adhesion and distribution.
  • To assess the suitability of immobilized EVs for super-resolution microscopy (SRM) and B-cell interaction assays.

Main Methods:

  • EVs were applied to borosilicate glass and quartz coverslips, with and without poly-L-lysine (PLL) pre-coating.
  • EV immobilization efficiency was assessed on different surfaces and under varying mounting media conditions for SRM.
  • Immobilized EVs were utilized in a B-cell interaction test to evaluate functional relevance.

Main Results:

  • Borosilicate glass coverslips demonstrated superior EV immobilization compared to quartz glass coverslips.
  • Poly-L-lysine (PLL) was not essential for EV retention but significantly improved their uniform distribution on borosilicate glass.
  • Standard laboratory materials, including borosilicate glass coverslips with or without PLL, are effective for EV immobilization in imaging.

Conclusions:

  • Borosilicate glass coverslips offer a cost-effective and efficient surface for immobilizing extracellular vesicles (EVs).
  • Poly-L-lysine (PLL) can enhance the uniformity of EV distribution, aiding in imaging and analysis.
  • These findings support the use of readily available lab materials for EV immobilization in advanced microscopy techniques.