Bradykinin antagonizes morphine-induced analgesia in rats

The intraventricular injection of 5 micrograms of bradykinin or Met-Lys-bradykinin in rats antagonized the increase in nociception threshold induced by morphine (5 micrograms i.c.v. or 10 mg/kg i.p.) in the tail flick test. The effect of the two kinins was prevented by atropine (30 mg/kg i.p.). The results suggest that bradykinin counteracts opiate activity in the central nervous system by enhancing acetylcholine release.