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Efficient Bioprocess for Mixed PET Waste Depolymerization Using Crude Cutinase.

Virender Kumar1, Reinhard Wimmer1, Cristiano Varrone1

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Summary

This study introduces a new bioprocess using crude enzymes from E. coli to break down mixed plastic waste, achieving high depolymerization rates for PET (polyethylene terephthalate) and demonstrating potential for industrial plastic recycling.

Keywords:
bioprocessdepolymerizationmixed PET waste

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Area of Science:

  • Biotechnology
  • Environmental Science
  • Biochemistry

Background:

  • Several plastic-degrading enzymes capable of depolymerizing polyethylene terephthalate (PET) have been identified.
  • Efficient depolymerization of mixed PET waste remains a challenge for industrial applications.

Purpose of the Study:

  • To develop and optimize a bioprocess for the depolymerization of mixed PET waste using crude, extracellularly expressed enzymes.
  • To evaluate the efficiency of different enzymes (FastPETase, LCC, LCCICCG) in breaking down various forms of PET waste.

Main Methods:

  • Screening of FastPETase, LCC, and LCCICCG enzymes for PET depolymerization of amorphous PET powder and films.
  • Optimization of enzyme conditions and cultivation of LCCICCG-producing E. coli in a bioreactor.
  • Application of crude LCCICCG supernatant for depolymerizing mixed PET waste in a bioreactor.

Main Results:

  • LCCICCG achieved up to 70% depolymerization of PET waste under optimized conditions without pH control.
  • Extracellular LCCICCG production in E. coli yielded 119 ± 5 mg L-1 protein with a specific activity of 1232 ± 18 U mg-1.
  • A bioprocess using crude LCCICCG achieved 78% and 50% yield for mixed PET waste (MW1 and MW2) in 60 hours at 62 °C.

Conclusions:

  • A cost-effective and efficient bioprocess for mixed PET waste depolymerization was successfully developed using crude LCCICCG.
  • The developed strategy shows significant potential for large-scale plastic waste treatment and recycling.
  • This approach offers an accessible method for PET depolymerization, reducing reliance on purified enzymes.