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Updated: Jun 2, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A novel CRISPR-Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive
Yu Qin1, Jia Li Xie1, Kai Mao1
1College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, P. R. China. zsj@swu.edu.cn.
Abstract:
Here, a CRISPR-Cas12a system with high trans-cleavage ability integrating a DNA nanochain formed by DNA tetrahedrons with a large molecular mass was employed to enhance the signal-to-background ratio of the fluorescence anisotropy method for achieving sensitive detection of hepatitis B virus DNA.
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