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Aptamer-Functionalized Platform for Selective Bacterial Isolation and Rapid RNA Purification Using Capture Pins.
Md Aminul Islam1, Rebecca Giorno2, Gergana G Nestorova2
1Molecular Sciences and Nanotechnology, Louisiana Tech University, Ruston, LA 71272, USA.
Sensors (Basel, Switzerland)
|April 28, 2025
Summary
This study introduces a novel piezoelectric platform for rapid bacterial lysis and RNA extraction. The device selectively captures and lyses E. coli, significantly enhancing RNA purification for diagnostics.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Biosensing
Background:
- Efficient bacterial lysis and RNA purification are critical for molecular diagnostics and biosensing.
- Existing methods often lack specificity or require complex procedures.
Purpose of the Study:
- To develop an integrated piezoelectric platform for selective bacterial capture, lysis, and RNA extraction.
- To enhance the enrichment of specific RNA molecules, such as 16S ribosomal RNA (rRNA).
Main Methods:
- A 3D-printed piezoelectric platform with gold-plated RNA capture pins (RCPs) functionalized with E. coli-specific aptamers.
- Utilized a 60 kHz piezoelectric transducer for bacterial cell wall disruption.
- Assessed bacterial lysis efficiency, RNA yield, and 16S rRNA enrichment.
Main Results:
- Demonstrated high specificity for E. coli over B. cereus.
- Achieved significant bacterial lysis, reducing viability to 33.68% in 15 minutes.
- Obtained a threefold enrichment of 16S rRNA and higher total RNA yield compared to sonication.
Conclusions:
- The integrated piezoelectric platform provides a rapid, selective, and label-free method for bacterial lysis and RNA extraction.
- Offers potential applications in clinical diagnostics and microbial biosensing by improving RNA purification and enrichment.
