Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
DNA Packaging00:58

DNA Packaging

Overview
Chromatin Packaging02:21

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The Nucleosome01:19

The Nucleosome

Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of low-level water addition for preparative chiral SFC.

Journal of chromatography. A·2026
Same author

Size-Selective FET Sensors Based on Semiconducting Single-Walled Carbon Nanotubes and Metal-Organic Frameworks.

ACS applied materials & interfaces·2026
Same author

Development of a population based patient cancer data warehouse from multiple electronic health record systems.

Health informatics journal·2026
Same author

Two hundred years of historical spawning and nursery data for coregonine fishes in the Laurentian Great Lakes.

Scientific data·2026
Same author

Interactions of the DNA Nanostructure with Silane-Based Self-Assembled Monolayers.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Intrinsic Wettability of Talc.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Metal-Organic Framework Multizyme Colloids with Joint Antioxidant and Protease Function.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Morphology Engineering of Co<sub>3</sub>O<sub>4</sub> via Cetyltrimethylammonium Bromide-Mediated ZIF-67 Synthesis for Efficient Photo-Assisted Electrooxidation of Methanol.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Speciation of Silanol Groups on Commercial Precipitated Silicas via IR Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Hydrogen Plasma-Driven Surface Defect Engineering of ZnO Nanorods: Correlating Electronic Structure and Photoelectrochemical Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Cooperative Self-Assembly of Nanoparticle-Encapsulating Hybrid Protein Cages.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

10.8K

DNA Nanostructure Deposition on Self-Assembled Monolayers.

Anumita Kumari1, Jason Smith1,2, Jonathan Cho1

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|April 28, 2025
PubMed
Summary
This summary is machine-generated.

DNA nanostructures remain stable on hydrophilic surfaces but deform on hydrophobic ones. Surface properties and washing affect DNA stability, highlighting interactions crucial for DNA nanotechnology applications.

More Related Videos

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.2K
Author Spotlight: Exploring Self-Assembled MOF-Polymer Composites
06:48

Author Spotlight: Exploring Self-Assembled MOF-Polymer Composites

Published on: June 14, 2024

1.5K

Related Experiment Videos

Last Updated: Jul 3, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
08:09

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates

Published on: May 9, 2014

10.8K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.2K
Author Spotlight: Exploring Self-Assembled MOF-Polymer Composites
06:48

Author Spotlight: Exploring Self-Assembled MOF-Polymer Composites

Published on: June 14, 2024

1.5K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Self-assembled monolayers (SAMs) are widely used substrates in nanotechnology.
  • Controlling the stability of DNA nanostructures on surfaces is critical for their applications.
  • Understanding surface-DNA interactions is key to advancing DNA nanotechnology.

Purpose of the Study:

  • To investigate the stability of DNA nanostructures deposited on both hydrophilic and hydrophobic SAMs.
  • To explore the influence of SAM surface properties on DNA nanostructure integrity.
  • To elucidate the factors governing DNA nanostructure stability on different substrates.

Main Methods:

  • Deposition of DNA nanostructures onto various SAMs (hydrophilic and hydrophobic).
  • Characterization of DNA nanostructure morphology and stability using microscopy techniques.
  • Analysis of the impact of postdeposition washing procedures on structural integrity.

Main Results:

  • DNA nanostructures maintained structural integrity on hydrophilic SAMs.
  • Significant deformation of DNA nanostructures was observed on highly hydrophobic SAMs.
  • Increased surface hydrophobicity correlated with increased DNA nanostructure deformation.
  • Postdeposition washing procedures were found to influence DNA nanostructure stability.

Conclusions:

  • The wettability of SAMs plays a critical role in the structural stability of deposited DNA nanostructures.
  • Deformation on hydrophobic surfaces is likely due to disrupted hydrogen bonding and interfacial tension during drying.
  • π-π stacking interactions may contribute to DNA nanostructure stabilization on SAMs.
  • This study expands potential substrates for DNA nanotechnology and emphasizes the need for understanding surface-DNA interactions.