Engineering Escherichia coli for Anaerobic Succinate Fermentation Using Corn Stover Hydrolysate as a Substrate
Haining Yang1,2, Yali Dong3
1School of Biological Engineering, Xinxiang University, Xinxiang 453003, P.R China.
Journal of Microbiology and Biotechnology
|April 28, 2025
Summary
This study enhances microbial succinate production by optimizing metabolic pathways and cofactor regeneration, achieving a 2.06-fold increase in titer. An IPTG-free system using corn stover hydrolysate significantly reduced fermentation costs.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Industrial Microbiology
Background:
- Succinic acid is a vital platform chemical with broad industrial applications.
- Current microbial succinate production faces challenges including low titer, cofactor imbalance, and high costs.
Purpose of the Study:
- To overcome limitations in microbial succinate synthesis.
- To enhance succinate production efficiency and reduce costs.
Main Methods:
- Engineered the reductive tricarboxylic acid (TCA) cycle and glucose uptake pathway.
- Overexpressed formate dehydrogenase to improve NADH regeneration.
- Implemented an oxygen-responsive biosensor to replace IPTG induction.
Main Results:
- Achieved a succinate titer of 4.31 g/l, a 2.06-fold increase over the original strain.
- Successfully produced 60.74 g/l succinate in a 5 L bioreactor using corn stover hydrolysate.
- Demonstrated significantly reduced fermentation costs by utilizing biomass hydrolysate.
Conclusions:
- The engineered strain shows high succinate titer and cost-effectiveness using lignocellulosic biomass.
- Metabolic engineering strategies effectively address bottlenecks in microbial succinate production.
- The developed system offers a sustainable and economical approach for industrial succinate manufacturing.
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