Bioactive Formulation and Bulk Allopurinol and Lycopene Estimation by UV Spectroscopy
Soumya Mishra1,2, Ranjit K Harwansh1, Rupa Mazumder2
1Department of Pharmacy, Institute of Pharmaceutical Research, GLA University, Mathura, 281406, India.
Drug Metabolism and Bioanalysis Letters
|April 29, 2025
Summary
A new UV spectrophotometric method accurately quantifies allopurinol and lycopene in bulk and dosage forms. This practical approach ensures reliable quality control with high sensitivity and linearity.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Development of a practical UV spectrophotometric method for quantifying allopurinol and lycopene.
- Focus on simplicity and applicability to both bulk and dosage forms.
Purpose of the Study:
- To establish a reliable and interference-free UV spectrophotometric method for allopurinol and lycopene quantification.
- To validate the method according to ICH standards for routine quality control.
Main Methods:
- Method development and validation using methanol and lycopene in phosphate buffer saline across various pH levels.
- Assessment of linearity, accuracy, precision, limit of quantification (LOQ), and limit of detection (LOD).
Main Results:
- High sensitivity and linearity observed within the 4-20 μg/ml concentration range, obeying Beer's law.
- Optimal absorbance wavelengths determined at 251 nm for lycopene and 471 nm for allopurinol.
- Excellent correlation coefficient (0.9994) indicating a strong linear relationship; LOQ and LOD values established.
Conclusions:
- The developed UV spectrophotometric method is effective and interference-free.
- The method is suitable for routine quality control of allopurinol and lycopene.
- Reliable quantitative measurements are achievable for both compounds.
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