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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Related Experiment Video

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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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From Genes to Pathways: A Curated Gene Approach to Accurate Pathway Reconstruction in Teleost Fish Transcriptomics.

Marcela Herrera1, Stefano Vianello2, Laurie Mitchell1

  • 1Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan.

Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
|April 29, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a manually curated gene list for teleost fish transcriptomics, improving data interpretation for non-model organisms. This resource aids researchers in understanding fish physiology and adaptation through targeted gene analysis.

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Area of Science:

  • Comparative genomics
  • Molecular biology
  • Aquatic biology

Background:

  • High-throughput sequencing generates vast data, challenging interpretation, especially for non-model organisms.
  • Automated analyses like Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) lack specificity for non-model species.
  • Teleost fish research requires specialized tools for accurate transcriptomic data analysis.

Purpose of the Study:

  • To develop a manually curated gene list specifically for teleost fish transcriptomics.
  • To enhance the specificity and reliability of gene enrichment analyses in non-model fish species.
  • To support research on teleost fish physiology, development, and adaptation.

Main Methods:

  • Manual curation of gene lists by interdisciplinary specialists.
  • Prioritization of genes based on established roles in teleost physiology and experimental evidence.
  • Focus on key biological processes including hormone signaling, metabolism, and osmoregulation.

Main Results:

  • A tailored gene list for teleost fish transcriptomics has been created.
  • The list includes genes critical for hormone signaling, metabolism, digestion, vision, and osmoregulation.
  • This resource offers a reliable starting point for teleost transcriptomic studies.

Conclusions:

  • The curated gene list improves the interpretation of transcriptomic data for teleost fish.
  • This resource streamlines gene selection and analysis for teleost research.
  • Collaborative refinement of the list will ensure its ongoing utility for the scientific community.