Related Experiment Video
Updated: May 8, 2026
Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal
Published on: October 11, 2019
Identification of characteristic genes in cutaneous squamous cell carcinoma based on weighted gene co-expression
Qipeng Xiao1, Pengfei Xu1, Wenjun Xu2
1Department of Dermatology, Affiliated Hospital of Jiujiang University, Jiujiang, Jiangxi, China.
Objective:
This study aims to identify characteristic genes associated with cutaneous squamous cell carcinoma (cSCC).
Methods:
Differentially expressed genes (DEGs) and hub genes in key module were identified using the limma package and weighted gene co-expression network analysis (WGCNA) in R software, respectively. The intersection of these genes was then subjected to LASSO regression to pinpoint characteristic genes. The correlation between immune cell infiltration and these characteristic genes was further elucidated using single-sample Gene Set Enrichment Analysis and Spearman correlation analysis.
Results:
A total of 113 DEGs were identified, along with their associated biological pathways. From this pool, five characteristic genes-ADH1B, CCL27, ID4, LRP4 and S100A9-were selected and validated. Immune infiltration analysis revealed significant correlations between these genes and various immune cell types, particularly with CCL27, ID4, LRP4 and S100A9.
Conclusion:
The identification of characteristic genes for cSCC provides valuable insights into its molecular mechanisms. The correlations between these genes and immune cell infiltration suggests their potential roles in tumor immunity.
