Related Experiment Video
Updated: Sep 6, 2026

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
Published on: December 13, 2012
A Method to Visualize Cell Proliferation of Arabidopsis thaliana: A Case Study of the Root Apical Meristem
J Irepan Reyes-Olalde1,2, Miguel Tapia-Rodríguez3, Vadim Pérez-Koldenkova4
1Laboratorio de Genética Molecular, Epigenética, Desarrollo y Evolución de Plantas, Instituto de Ecología Universidad Nacional Autónoma de México, Ciudad Universitaria Ciudad de México México.
Abstract:
Plant growth and development rely on a delicate balance between cell proliferation and cell differentiation. The root apical meristem (RAM) of Arabidopsis thaliana is an excellent model to study the cell cycle due to the coordinated relationship between nucleus shape and cell size at each stage, allowing for precise estimation of the cell cycle duration. In this study, we present a method for high-resolution visualization of RAM cells. This is the first protocol that allows for simultaneous high-resolution imaging of cellular and nuclear stains, being compatible with DNA replication markers such as EdU, including fluorescent proteins (H2B::YFP), SYTOX DNA stains, and the cell wall stain SR2200. This protocol includes a clarification procedure that enables the acquisition of high-resolution 3D images, suitable for detailed subsequent analysis.

