Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From bulk parameters to catalytic activity: descriptors for nonprecious metal electrocatalysts for water electrolysis.

Chemical communications (Cambridge, England)·2026

Same author

Computational analysis of water coverage formation on Pt(111) surface under humid conditions with the effect of applied voltage.

Physical chemistry chemical physics : PCCP·2025

Same author

Highly phase-separated alternating copolymer of alkyl vinyl ether and sulfonic acid group-containing trifluoro vinyl ether.

RSC advances·2025

Same author

Effect of organic ligands in iron-based metal-organic frameworks on oxygen evolution electrocatalysis: conventional inorganic solids <i>vs.</i> organic-inorganic hybrid materials.

Chemical communications (Cambridge, England)·2025

Same author

Ultrasmall Manganese Nanospinels Produced via an Alcohol Reduction Method and Their Electrocatalytic Oxygen Evolution Reactivity.

ACS applied materials & interfaces·2025

Same author

Electrocatalysts for Hydrogen Production - Catalyst Design Strategies Based on Crystal Structures of Multimetal Oxides.

Chemical record (New York, N.Y.)·2025

Search research articles

Related Experiment Video

Updated: May 9, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

A membrane-based immunosensor enabling high antifouling performance and sensitive molecular recognition.

Hiroki Yamashita¹, Hiroto Okuyama¹, Takeo Yamaguchi¹

¹Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Kanagawa 226-8501, Japan. yamag@res.titech.ac.jp.

|April 29, 2025

Summary

This study presents a novel porous membrane immunosensor that overcomes the trade-off between antifouling and sensitivity. The new design achieves high sensitivity and effective antifouling for improved diagnostic applications.

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Related Experiment Videos

Last Updated: May 9, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Biosensing Technology
Surface Chemistry

Background:

Non-targeted biomolecule fouling on sensing surfaces significantly degrades immunosensor performance.
Hydrophilic polymers offer antifouling but can reduce antibody density, decreasing sensitivity.
A balance between antifouling and high sensitivity is crucial for practical immunosensing platforms.

Purpose of the Study:

To develop a novel porous-membrane-based immunosensor that achieves both high sensitivity and effective antifouling.
To mitigate signal reduction caused by antifouling moieties through antibody densification within submicron pores.
To optimize the receptor/antifouling moiety ratio using numerical modeling.

Main Methods:

Fabrication of a porous-membrane-based immunosensor.
Incorporation of antifouling moieties and antibody densification within submicron pores.
Numerical modeling to determine the optimal ratio of receptor to antifouling moiety.
Performance evaluation using interleukin-6 (IL-6) detection in artificial saliva and serum.

Main Results:

The designed immunosensor successfully integrated antifouling properties with high sensitivity.
Antibody densification in submicron pores effectively mitigated signal loss associated with antifouling.
The sensor demonstrated excellent antifouling capabilities and high sensitivity for IL-6 detection.
Achieved limits of detection of 4.8 pg mL-1 in artificial saliva and 1.2 pg mL-1 in serum.

Conclusions:

The porous-membrane-based immunosensor effectively addresses the challenge of achieving both high sensitivity and antifouling.
The strategy of antibody densification in submicron pores is a promising approach for enhancing immunosensor performance.
The developed sensor shows significant potential for practical diagnostic applications, particularly in complex biological matrices.