Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantum Well-Enhanced Plasmonic Substrate to Enhance Spontaneously Blinking Fluorescence for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same author

Microplastic and microcystin in tropical drinking water reservoir: pollution characteristics and human health risk assessment.

Environmental monitoring and assessment·2025
Same author

Microplastics in wastewater and the role of local wastewater treatment stations in controlling microplastic pollution: a case study from Vietnam.

Environmental monitoring and assessment·2025
Same author

Temporal-Focusing Multiphoton Excitation Single-Molecule Localization Microscopy Using Spontaneously Blinking Fluorophores.

Angewandte Chemie (International ed. in English)·2024
Same author

A Fluorescent Vector of Carbon Dot to Deliver Rab13 and Rab14 Plasmids for Promoting Neurite Outgrowth.

ACS applied bio materials·2023
Same author

Microplastics in sediments from urban and suburban rivers: Influence of sediment properties.

The Science of the total environment·2023

Related Experiment Video

Updated: Jun 12, 2026

Nanofabrication of Gate-defined GaAs/AlGaAs Lateral Quantum Dots
15:47

Nanofabrication of Gate-defined GaAs/AlGaAs Lateral Quantum Dots

Published on: November 1, 2013

16.1K

Single-Base Detection of DNA with Simplified Steps on InGaN Quantum Wells.

Thi Anh Nguyet Nguyen1, Ching-Lung Luo1, Fan-Ching Chien1

  • 1Department of Optics and Photonics, National Central University, Chung-Li, Taoyuan 32001, Taiwan.

The Journal of Physical Chemistry. B
|April 29, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a simplified DNA detection method using surface-enhanced Raman spectroscopy (SERS) for rapid and accurate genetic analysis. The novel biochip achieves single-molecule sensitivity, accelerating disease prevention through efficient DNA testing.

More Related Videos

Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping
14:58

Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping

Published on: June 3, 2015

14.4K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.1K

Related Experiment Videos

Last Updated: Jun 12, 2026

Nanofabrication of Gate-defined GaAs/AlGaAs Lateral Quantum Dots
15:47

Nanofabrication of Gate-defined GaAs/AlGaAs Lateral Quantum Dots

Published on: November 1, 2013

16.1K
Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping
14:58

Silicon Metal-oxide-semiconductor Quantum Dots for Single-electron Pumping

Published on: June 3, 2015

14.4K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.1K

Area of Science:

  • Biotechnology
  • Spectroscopy
  • Genetics

Background:

  • DNA testing is crucial for assessing genetic disorder risk and disease prevention.
  • Current DNA testing methods are often tedious, involving multiple molecular modification steps.
  • There is a need for faster, more accurate DNA detection techniques.

Purpose of the Study:

  • To develop a simplified DNA detection tactic that bypasses traditional complex steps.
  • To achieve rapid, high-sensitivity DNA detection using a novel biochip.
  • To demonstrate the capability of capturing submicron images of dilute DNA.

Main Methods:

  • Utilized surface-enhanced Raman spectroscopy (SERS) with InGaN quantum wells and Al nanospheres.
  • Developed a biochip that exposes nucleotides to a SERS hot surface.
  • Skipped surface functionalization, fluorescent labeling, and probe immobilization.

Main Results:

  • Achieved single-molecule sensitivity in DNA detection.
  • Captured a wide-field submicron image of dilute DNA (1 x 10^-9 M) in 3.5 minutes.
  • Demonstrated a simplified DNA detection tactic with enhanced nucleotide exposure.

Conclusions:

  • The developed SERS biochip offers a simplified and highly sensitive approach for DNA detection.
  • This method significantly reduces the complexity and time required for DNA analysis.
  • The technology holds potential for accelerating the prevention of genetic disorders and other diseases.