Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dehydroepiandrosterone (DHEA) and Dehydroepiandrosterone Sulfate (DHEA-S) in Broiler Plasma: Analytical Validation of Immunoassays and Comparative Overview Across Avian Species.

Animals : an open access journal from MDPI·2026

Same author

Nrf2 Levels in Human Semen and Spermatozoa: Association with F<sub>2</sub>-Isoprostanes as Markers of Lipid Peroxidation.

International journal of molecular sciences·2026

Same author

D-Ribose Glycation of Human High-Density Lipoprotein: Structural and Functional Alterations.

International journal of molecular sciences·2026

Same author

Sperm Antioxidant Capacity Discriminates Between Fertile and Infertile Men and Is Strictly Related to Lipid Peroxidation and Lipid Mediator Production.

Biology·2026

Same author

Detection of viruses associated with bovine respiratory disease complex in samples collected from Albanian cattle during 2022/2023.

Access microbiology·2026

Same author

Serum kisspeptin levels in pregnant and non-pregnant diestrus bitches: a pilot study.

Frontiers in veterinary science·2026

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

In Vitro Effects of Lipopolysaccharide on Rabbit Sperm: Toll-like Receptor 4 Expression, Motility, and Oxidative

Alda Quattrone¹, Nour Elhouda Fehri¹, Stella Agradi²

¹Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.

Antioxidants (Basel, Switzerland)

|April 29, 2025

Summary

Lipopolysaccharide (LPS) at doses of 400 µg/mL and higher impairs rabbit sperm motility. This study also identified Toll-like receptor 4 (TLR4) on rabbit sperm, establishing a threshold LPS dose for future research.

Keywords:

CASA system TLR4 in vitro lipopolysaccharide male infertility malondialdehyde oxidative stress rabbit buck sperm motility toll-like receptor

More Related Videos

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Area of Science:

Reproductive Biology
Immunology
Toxicology

Background:

Inflammation induced by lipopolysaccharide (LPS) is known to affect sperm function.
The specific impact of LPS on ejaculated rabbit sperm and the presence of Toll-like receptor 4 (TLR4) on these cells remain uninvestigated.

Purpose of the Study:

To determine the LPS concentration that negatively impacts in vitro sperm motility in rabbits.
To identify TLR4 localization on rabbit spermatozoa.
To evaluate seminal plasma malondialdehyde (MDA) levels as an indicator of oxidative stress.

Main Methods:

Sperm motility was assessed using computer-assisted sperm analysis (CASA) after incubation with varying LPS concentrations (0–800 µg/mL) over 4 hours.
Toll-like receptor 4 (TLR4) localization was determined using immunofluorescence.
Malondialdehyde (MDA) levels were measured via the thiobarbituric acid reactive substances (TBARS) assay.

Main Results:

LPS doses of 400 µg/mL and above significantly reduced sperm motility parameters (progressive, non-progressive, and curvilinear velocity) and increased static spermatozoa.
Receiver operating characteristic (ROC) analysis indicated a threshold LPS dose of 300 µg/mL for motility decline.
Immunofluorescence confirmed TLR4 presence in the midpiece of rabbit sperm, with increased labeling after LPS exposure. MDA levels were not significantly affected by LPS treatment.

Conclusions:

This study is the first to identify TLR4 on rabbit spermatozoa.
A threshold LPS dose of 300 µg/mL was established for impairing rabbit sperm motility in vitro.
LPS-induced inflammation does not appear to increase oxidative stress in rabbit seminal plasma, as indicated by MDA levels.