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Updated: May 15, 2025

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Oxo-Hydrazyl as a Substitute for the Stable Free Radicals Employed in Measuring Total Antioxidant Activity
1Faculty of Chemistry, University of Bucharest, Panduri 90, 050663 Bucharest, Romania.
Abstract:
Numerous standardized methods for evaluating antioxidant capacity are available, some of the most used methods in this regard employ stable free radicals, like 2,2-diphenyl-1-picrilhydrazyl free radical (DPPH·) or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid cation radical (ABTS·+). However, some challenges can arise in taking correct and reproducible measurements due to the well-known unspecific reactivity of these free radicals. In pursuit of improving and expanding such methods, in this work is proposed a highly intense colored zwitterionic derivative of the DPPH· free radical, as a replacement for DPPH· and ABTS·+ derivatives. A discussion and comparison of the recognized methods are presented, demonstrating the very good potential of this non-radical compound.
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