Hydrodynamic confinement of bacteria within intestinal folds

The gut microbiota significantly influence host health by impacting metabolism, immune function and development. Understanding bacterial behaviours in intestinal folds is crucial owing to their role in biofilm formation, which protects bacteria from immune responses and antibiotics and is associated with colorectal cancer. In this study, we observed the behaviours of Escherichia coli bacteria in the intestinal folds of zebrafish larvae (Danio rerio). It is found that E. coli swims in the intestinal folds for extended periods and is confined in a groove on the wall. In order to clarify the mechanism of the confinement, we further performed numerical simulations using a boundary element method. Our simulations demonstrate that bacterial movement in the groove is constrained by hydrodynamic and steric forces. The groove configuration significantly influences bacterial confinement, with bacteria in a deep groove escaping more easily in the presence of background flow. Based on the aggregation rate of E. coli in the intestinal folds of zebrafish larvae, it is indicated that the groove trapping significantly reduces cell flux away from the wall. These findings enhance our understanding of bacterial accumulation and biofilm formation in the gut, with implications for other environments with geometric constraints.