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Isolation of Staphylococcus aureus clumping factor.

F Espersen, I Clemmensen, V Barkholt

    Infection and Immunity
    |September 1, 1985
    PubMed
    Summary
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    Researchers isolated a key component from Staphylococcus aureus, likely the clumping factor, which binds to fibrinogen and inhibits bacterial clumping. This finding aids in understanding Staphylococcus aureus interactions.

    Area of Science:

    • Microbiology
    • Immunochemistry
    • Biochemistry

    Background:

    • Staphylococcus aureus is a significant human pathogen.
    • Bacterial surface components mediate host-pathogen interactions.
    • Understanding these interactions is crucial for developing therapeutic strategies.

    Purpose of the Study:

    • To isolate and characterize immunochemically identical components from Staphylococcus aureus.
    • To investigate the role of these components in bacterial clumping and fibrinogen binding.
    • To identify the Staphylococcus aureus clumping factor.

    Main Methods:

    • Affinity chromatography using fibrinogen-linked Sepharose 4B.
    • Immunoelectrophoresis and Western blot analysis.
    • Gel filtration and SDS-PAGE for molecular mass determination.

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  • Chemical analysis for carbohydrate content.
  • Crossed immunoelectrophoresis for fibrinogen binding assay.

    • Main Results:

    • Isolation of an immunochemically identical component from five Staphylococcus aureus strains.
    • The component was found in whole cells, cell walls, and extracellular products, but not in L-forms or Staphylococcus epidermidis.
    • Molecular mass determined as 420,000 (gel filtration) and 360,000 (SDS-PAGE), with approximately 20% carbohydrate content.
    • The isolated component bound to human fibrinogen and inhibited fibrinogen-induced staphylococcal clumping.

    Conclusions:

    • The purified component is immunochemically identical across multiple Staphylococcus aureus strains.
    • The component's ability to bind fibrinogen and inhibit clumping strongly suggests it is the Staphylococcus aureus clumping factor.
    • This identification provides insights into the mechanisms of Staphylococcus aureus adhesion and pathogenesis.