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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
High oxygen-modified atmosphere packaging induces a viable but non-culturable state of Campylobacter jejuni in
Ayaka Okada1,2,3, Md Jannat Hossain1,2,4, Aimi Fuyuki2,5
1Laboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Abstract:
The high oxygen conditions (80% oxygen and 20% carbon dioxide) of oxygen-modified atmosphere packaging (high O2 MAP) maintain the quality of meat. This study investigates the cultivability and viability of Campylobacter jejuni in a high O2 MAP environment, with different strains investigated to examine whether cultivability is affected by aerotolerance. C. jejuni strains spiked chicken meat was exposed to high O2 MAP and assessed using culture and viability-digital PCR. The results indicated suppression of the cultivability of C. jejuni under high O2 MAP conditions, with evidence suggesting that this may not be due to aerotolerance. However, the viability of C. jejuni was not affected, indicating that bacterial cells might have been induced to a viable but non-culturable state.
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