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Related Experiment Videos

C56 formation in the reaction mixture of isolated complement components through the classical complement pathway.

H Kitamura, M Tsuboi, K Nagaki

    International Archives of Allergy and Applied Immunology
    |January 1, 1985
    PubMed
    Summary

    This study investigated the complement system

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    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • The complement system is crucial for innate and adaptive immunity.
    • Understanding complement-mediated hemolysis is vital for immunological research.

    Purpose of the Study:

    • To elucidate the mechanism of hemolysis by isolated human complement components.
    • To identify the lytic factor generated from C1s-C6.

    Main Methods:

    • Hemolysis assays using various erythrocytes (guinea pig, human, sheep).
    • Characterization of a generated lytic factor through component interactions and biochemical analyses.
    • Gel filtration to compare elution volumes of the lytic factor and C56.

    Main Results:

    • Guinea pig erythrocytes were most susceptible to lysis.
    • A lytic factor generated from C1s-C6 was identified and characterized.
    • This factor demonstrated properties similar to C56, including C7-dependent binding to erythrocytes.

    Conclusions:

    • The lytic factor is likely C56, generated during the reaction of C1s-C6.
    • This C56 intermediate, along with C7, forms an EC567 complex.
    • The EC567 complex is then lysed by C8 and C9, completing the membrane attack complex formation.

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