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In Vitro multinucleated giant cell-like cells formation: An observation
W X Kok1, S K Cheong2, P P Leong3
1Postgraduate Research Laboratories, M. Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman (UTAR), Kajang, Malaysia.
The Malaysian Journal of Pathology
|April 30, 2025
Summary
Multinucleated giant cells (MGCs) emerged from umbilical cord blood cells in a novel methylcellulose culture. This finding offers a new model for studying monocytic-lineage cell development and MGC formation in vitro.
Area of Science:
- Cell Biology
- Hematopoiesis
- Immunology
Background:
- Multinucleated giant cells (MGCs) are associated with chronic inflammation.
- Molecular mechanisms of MGC adhesion and signaling pathways remain largely unknown.
- Current in vitro models using isolated monocytes/macrophages do not fully replicate in vivo MGCs.
Purpose of the Study:
- To report the novel emergence of MGCs from umbilical cord blood in a modified methylcellulose culture.
- To propose an alternative model for monocytic-lineage cell development.
Main Methods:
- Utilized a 10-day colony-forming unit (CFU) assay with umbilical cord blood mononuclear cells in methylcellulose culture.
- Observed the formation of adherent epithelioid-like clusters.
Main Results:
- Adherent clusters yielded monocytic-like cells and multinucleated giant cells (MGCs).
- MGCs exhibited characteristic multinucleated morphology.
- Cell emergence occurred during the later stages of the culture timeline.
Conclusions:
- This observation provides a reference for future cell culture studies.
- Presents a potential alternative model for monocytic-lineage cell development.
- Suggests an intermediary stage in differentiation, diverging from the linear hematopoietic hierarchy.
