JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Period 1 (PER1): A novel glucocorticoid-responsive gene involved in cortisol-induced proliferation of androgen-independent human prostate cancer DU145 cells

  • 0Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Fukumuro, Miyagino-ku, Sendai, Miyagi, Japan.
Histology and Histopathology +

|

April 30, 2025

|

April 30, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Glucocorticoid receptor (GR) stimulation by dexamethasone (DEX) increases Period 1 (PER1) gene expression in prostate cancer cells. PER1 products are crucial for DEX-induced cell proliferation in androgen-independent prostate cancer.

Area Of Science

  • Oncology
  • Molecular Biology
  • Endocrinology

Background

  • The glucocorticoid receptor (GR) plays a role in prostate cancer progression.
  • Understanding GR's influence on androgen-independent prostate cancer is crucial for treatment strategies.

Purpose Of The Study

  • To investigate the effect of cortisol on genes regulating cell proliferation in androgen-independent human prostate cancer DU145 cells.
  • To identify specific genes induced by glucocorticoids in prostate cancer.

Main Methods

  • Gene expression profiling using RT² Profile PCR Array System.
  • Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR).
  • Immunoblot analysis and short hairpin RNA (shRNA) knockdown.

Main Results

  • Dexamethasone (DEX) significantly increased the expression of Period 1 (PER1) in DU145 cells.
  • PER1 products were found to be involved in DEX-induced proliferation of DU145 cells.
  • PER1 acts as a glucocorticoid-responsive gene in this context.

Conclusions

  • PER1 is a key gene regulated by the glucocorticoid receptor (GR) in androgen-independent prostate cancer.
  • PER1 plays a significant role in GR-stimulated DU145 cell proliferation.
  • PER1 may be a potential therapeutic target in GR-related prostate cancer.

Related Concept Videos

Periodic Classification of the Elements 04:00

43.1K

The periodic table arranges atoms based on increasing atomic number so that elements with the same chemical properties recur periodically. When their electron configurations are added to the table, a periodic recurrence of similar electron configurations in the outer shells of these elements is observed. Because they are in the outer shells of an atom, valence electrons play the most important role in chemical reactions. The outer electrons have the highest energy of the electrons in an atom...

Effective Value of a Periodic Waveform 01:07

454

The concept of effective value, the root mean square (RMS) value, is crucial in understanding electrical circuits and power delivery. This idea emerges from the necessity to measure the effectiveness of a voltage or current source in supplying power to a resistive load.
The effective value of a periodic current represents the direct current (DC) that conveys the same average power to a resistor as the periodic current itself. This concept is crucial when assessing AC circuits. To determine the...

The Periodic Table 03:25

75.2K

As early chemists discovered more elements, they realized that various elements could be grouped by their similar chemical behaviors. One such grouping includes lithium (Li), sodium (Na), and potassium (K). All of these elements are shiny, conduct heat and electricity well, and have similar chemical properties. A second grouping includes calcium (Ca), strontium (Sr), and barium (Ba), which also are shiny, good conductors of heat and electricity, and have chemical properties in common. However,...

The Periodic Table and Organismal Elements 00:57

172.2K

Overview

Elements are the smallest units of matter that cannot be broken down further by chemical processes. There are 118 known elements, but not all of these are naturally-occurring, and fewer still are essential for life. Living matter is composed primarily of carbon, nitrogen, hydrogen, and oxygen, with smaller amounts of other elements like calcium, phosphorus, potassium, and sulfur. Other elements are also necessary for life but only in trace amounts.

The Periodic Table Provides...

Properties of Laplace Transform-II 01:16

153

Time differentiation, convolution, integration, and periodicity are fundamental concepts in analyzing functions and signals over time. Each concept provides a unique perspective on how functions evolve, interact, and repeat, offering essential tools for various scientific and engineering applications.
Time differentiation involves analyzing the rate of change of a function over time. Mathematically, it is the derivative of a function with respect to time. This concept can be likened to tracking...

Piaget's Stage 1 of Cognitive Development 01:14

553

The sensorimotor stage, the initial phase of Jean Piaget's theory of cognitive development, spans the first two years of a child's life. During this period, infants actively engage with their surroundings, building cognitive awareness through direct interaction with the world. This interaction is primarily based on sensory perception and motor actions, allowing infants to gradually understand basic physical properties and predict how objects interact within their environment.