Related Experiment Video
Updated: May 9, 2025
08:10
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019
8.3K
A RFLP 1-4-3 L1C Variant of PRRSV-2 Isolated in Sichuan Province, China: Genetic Characterization and Pathogenicity
Li-Shuang Deng1, Zhi-Jie Jian1, Yuan-Meng Wang1
1College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Transboundary and Emerging Diseases
|April 30, 2025
Summary
A new L1C variant of Porcine reproductive and respiratory syndrome virus (PRRSV), SCABTC-202302, shows high recombination potential and causes significant lung and lymph node damage in piglets. This study details its genetic evolution and pathogenicity for improved PRRSV control.
Area of Science:
- Veterinary Virology
- Animal Pathology
- Molecular Epidemiology
Background:
- Porcine reproductive and respiratory syndrome virus (PRRSV) causes substantial economic losses in the swine industry.
- The L1C (lineage 1.8/NADC30-like) PRRSV variant has shown high recombination potential and pathogenicity since its emergence in China.
- Understanding novel PRRSV strains is crucial for disease management.
Purpose of the Study:
- To characterize a novel, naturally occurring L1C PRRSV recombinant variant (SCABTC-202302).
- To investigate its genetic evolution, recombination events, pathogenicity, and host immune response.
- To provide insights for developing effective PRRSV vaccines and control strategies.
Main Methods:
- Phylogenetic analysis of the ORF5 gene and whole-genome sequencing.
- Restriction fragment length polymorphism (RFLP) pattern analysis.
- Pathogenicity assessment in piglets, including viremia, inflammatory markers, and histopathology.
- Recombination analysis to identify parent strains.
Main Results:
- The SCABTC-202302 strain was classified as lineage 8.7 (ORF5) and L1C (whole-genome).
- A 131 amino acid deletion in NSP2 and specific ORF5 mutations were identified.
- Recombination analysis indicated NADC30 as the primary parent, with JXA1 contributing to ORF2-ORF7.
- Infection led to lung and lymph node damage, sustained viremia, and elevated inflammatory factors.
Conclusions:
- The SCABTC-202302 strain represents a significant L1C recombinant with notable genetic alterations and pathogenicity.
- Detailed characterization aids in understanding L1C PRRSV evolution and virulence.
- Findings support the development of targeted vaccines and control measures against emerging PRRSV strains.
Related Concept Videos
00:57Viral Recombination
23.1K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.1K
01:05Single Nucleotide Polymorphisms-SNPs
13.6K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
13.6K
00:36Viral Mutations
32.0K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.0K