A Fluorescent Immunochromatography Test Strip for the Rapid Identification of SVV and FMDV

Seneca Valley virus (SVV) and foot-and-mouth disease virus (FMDV) belong to the Picornaviridae family, which can cause similar symptoms. After infection, pigs will develop fever; loss of appetite; blister lesions on the skin and mucous membrane of the mouth, nose, and hoof; and other similar diseases, and the spread is very fast, causing major economic losses to the pig industry. Therefore, a rapid, accurate, and sensitive diagnostic method is necessary to enable rapid prevention and control measures for preventing the spread of these diseases. Here, a fluorescent immunochromatography test strip, using Eu-doped fluorescence beads and monoclonal antibody, was developed for the simultaneous determination of FMDV and SVV. The test process for the assay could be completed in 12 min, which avoided the time cost of the current methods for FMDV/SVV detection. Under optimized conditions, the limit of detection of SVV is 5 × 10⁴ PFU/mL, and that of FMDV is 5 × 10⁴ PFU/mL under the Fluorescence Immunoassay Analyzer. Our assay results showed a good linear correlation with RT-PCR installed in the clinical laboratory. The species design has a promising application prospect in the surveillance and control of the outbreak of idiopathic blister.