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Ischemic Stroke l: Introduction

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Updated: Jun 10, 2026

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

Decoding immune cell dynamics in ischemic stroke: insights from single-cell RNA sequencing analysis.

Yating Lan¹, Chun Zou², Feiyu Nong²

¹Department of Neurology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Frontiers in Aging Neuroscience

|April 30, 2025

Summary

Phagocytosis in ischemic stroke (IS) cell types significantly impacts disease progression. This study used single-cell RNA sequencing to reveal cellular mechanisms and potential therapeutic targets for IS.

Keywords:

cell communication differential gene expression enrichment analysis immune cells ischemic stroke phagocytosis single cell analysis

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Author Spotlight: Investigating the Complex Immune Response Following Brain Ischemia

Author Spotlight: Investigating the Complex Immune Response Following Brain Ischemia

Published on: July 26, 2024

Area of Science:

Neuroscience
Immunology
Genomics

Background:

Ischemic stroke (IS) is a major cause of adult disability globally.
The complex inflammatory processes in IS present challenges in understanding its pathology.
Phagocytosis is crucial for clearing neurotoxic debris during inflammatory responses in IS.

Purpose of the Study:

To investigate cell types and molecular processes in IS using bioinformatics.
To analyze single-cell RNA sequencing (scRNA-seq) data for IS.
To identify key cellular players and pathways in IS pathogenesis.

Main Methods:

Analysis of scRNA-seq data from healthy controls and IS patients.
Utilized Uniform Manifold Approximation and Projection (UMAP) for cell composition analysis.
Performed functional enrichment analysis and predicted cell developmental trajectories.
Employed iTALK to identify ligand-receptor interactions in the IS immune microenvironment.

Main Results:

Identified four distinct cell types and subclusters with significant differential gene expression in IS.
Found enriched phagocytosis in IS-associated cell types.
Observed altered differentiation trajectories in IS subpopulations.
Discovered multiple receptor-ligand axes, indicating complex immune microenvironment interactions.

Conclusions:

Phagocytosis in IS cell types is a critical factor influencing disease progression.
Predicted cell differentiation trajectories provide insights into IS pathogenesis.
Findings offer potential therapeutic targets for IS intervention.