JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Evaluation of the Structural, Biological, and Bone Induction Properties of Sol-Gel-Derived Lithium-Doped 68S Bioactive Glass-An in Vitro Study on Human Dental Pulp Stem Cells

  • 0Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran.
Clinical and Experimental Dental Research +

|

April 30, 2025

|

April 30, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Lithium-containing bioactive glass (BAG) enhances human dental pulp stem cell proliferation, differentiation, and mineralization. This biocompatible material shows promise for bone and dental tissue engineering.

Area Of Science

  • Biomaterials Science
  • Regenerative Medicine
  • Bioceramics

Background

  • Calcium silicate-based bioactive glasses (BAGs) promote hydroxyapatite (HA) formation and ion release.
  • Sol-gel synthesis offers precise control over BAG composition and properties.
  • Incorporating lithium (Li) into BAG may enhance its biological activity.

Purpose Of The Study

  • To synthesize and characterize a sol-gel-derived 68S BAG with 5 mol.% lithium (BGLi5).
  • To evaluate the structural, biological, and osteoinductive properties of BGLi5 using human dental pulp stem cells (hDPSCs).
  • To assess the antimicrobial efficacy of BGLi5 against Streptococcus mutans (SM).

Main Methods

  • Sol-gel synthesis of lithium-containing (BGLi5) and lithium-free (BG) 68S BAG.
  • Structural analysis (FESEM, FTIR) and hydroxyapatite (HA) formation assessment.
  • In vitro biological evaluation using hDPSCs (viability, ALP activity, mineralization) and antimicrobial testing against SM.

Main Results

  • HA crystal formation was confirmed in BGLi5 by Day 3 and BG by Day 7.
  • Both BG and BGLi5 enhanced hDPSC viability, proliferation, and mineralization.
  • BGLi5 significantly increased alkaline phosphatase (ALP) activity and mineralization, especially with differentiation medium.
  • Both materials exhibited significant antimicrobial activity against Streptococcus mutans.

Conclusions

  • Sol-gel derived 68S BAG with 5 mol.% Li (BGLi5) is biocompatible and enhances hDPSC proliferation, differentiation, and mineralization.
  • BGLi5 combined with differentiation medium synergistically promotes osteogenic differentiation and mineralization.
  • BGLi5 is a promising candidate for dental and bone tissue engineering applications.

Keywords: