Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Impact of Cooling Curve Type on Sperm DNA Integrity During Cryopreservation.

JBRA assisted reproduction·2026
Same author

Extracellular vesicles derived from dental mesenchymal stem cells for regenerative medicine: a scoping review.

Molecular biology reports·2025
Same author

Stress-inducible phosphoprotein 1 (STIP1) is a critical stemness regulator in mouse embryonic stem cells and early mammalian development.

Communications biology·2025
Same author

PD-L1 downregulation by carbonic anhydrase IX immunotherapy prompts immune checkpoint blockade in renal cell carcinoma.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models.

Genes·2024
Same author

<i>In vitro</i> culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax.

JBRA assisted reproduction·2024

Related Experiment Video

Updated: May 9, 2025

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

1.6K

Development and validation of a sperm-freezing device created using 3D printer technology.

Vera Lucia Lângaro Amaral1, Gabriela Reif1, Rafael Alonso Salvador1

  • 1Universidade do Vale do Itajaí, Itajaí, Santa Catarina, Brazil.

JBRA Assisted Reproduction
|April 30, 2025
PubMed
Summary

A new 3D-printed device offers reproducible and safe semen cryopreservation. This 3D-printed prototype ensures consistent cooling and comparable sperm recovery rates to traditional methods.

Keywords:
3D printercryopreservationliquid nitrogen vaporreproducibilitysperm

More Related Videos

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

11.8K
Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding
10:43

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

Published on: June 27, 2014

19.8K

Related Experiment Videos

Last Updated: May 9, 2025

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

1.6K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

11.8K
Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding
10:43

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

Published on: June 27, 2014

19.8K

Area of Science:

  • Reproductive biology
  • Biomedical engineering
  • Cryopreservation technology

Background:

  • Semen cryopreservation is crucial for assisted reproduction.
  • Standardized and reproducible freezing protocols are essential for maintaining sperm viability.
  • Current methods may lack consistency in cooling rates and handling safety.

Purpose of the Study:

  • To develop and evaluate a novel 3D-printed prototype for holding semen straws during cryopreservation.
  • To assess the safety, reproducibility, and effectiveness of the 3D-printed prototype compared to conventional supports.

Main Methods:

  • A 3D-printed prototype and an expanded polyethylene (EPS) support were used to hold ten semen straws each.
  • Temperature monitoring was performed using thermocouples within straws during freezing in liquid nitrogen vapor.
  • Sperm parameters including motility, vitality, and DNA fragmentation were analyzed before and after freezing.

Main Results:

  • The 3D-printed prototype demonstrated highly reproducible temperature cooling curves.
  • The prototype material proved durable, withstanding over 300 freezing cycles without degradation.
  • Sperm recovery rates for motility, vitality, and DNA integrity were comparable between the 3D-printed prototype and the EPS support.

Conclusions:

  • The 3D-printed prototype facilitates homogeneous, quantifiable, and reproducible cooling in liquid nitrogen vapor.
  • The device enhances safe handling of semen straws and provides explicit freezing conditions.
  • The prototype offers improved intra-operator and inter-laboratory reproducibility for sperm cryopreservation.