Enhancing simultaneous nitrogen and phosphorus removal from municipal wastewater using micron zeolite powder carrier...

This study developed a novel wastewater treatment process for efficient nitrogen and phosphorus removal using micron zeolite powder carriers and hydrocyclone separator. Under anaerobic/intermittent aeration, the total nitrogen and phosphorus removal efficiencies reached 85.2 ± 1.9 % and 78.9 ± 3.4 %, respectively, significantly outperforming conventional activated sludge system. High specific surface area and porosity of zeolite powder facilitated microbial aggregation and biofilm formation, resulting in an average sludge size of 125.3 ± 5.3 μm. The combination of powder carriers and hydrocyclone separators resulted in the differentiated distribution of functional microorganisms. Denitrifying bacteria, such as norank_Comamonadaceae (4.34 %), norank_AKYH767 (1.90 %), and Candidatus_Microthrix (2.61 %), were enriched in biofilm, while nitrifying bacteria and polyphosphate-accumulating organisms predominated in floc. Functional gene abundance related to denitrification and phosphorus removal was significantly upregulated. Correlation network analysis revealed enhanced microbial cooperation, improving the functionality and stability of community. This study offers the potential pathway for efficient nitrogen and phosphorus removal from municipal wastewater.