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Published on: September 21, 2021
Eremophilane sesquiterpenes from agarwood of Aquilaria agallocha with neuroprotective effect
Yanqiao Xie1, Siyu Zhang1, Tianqi Chen1
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory of Standardization of Chinese Medicines, The SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Abstract:
A total of 12 previously undescribed eremophilane-type sesquiterpenes agalleremonols A-L (1-12) were isolated from the agarwood of Aquilaria agallocha Roxb., and their structures were established based on extensive spectroscopic analyses. The absolute configurations of 1, 4, 5, and 12 were confirmed using single-crystal X-ray analysis, and the others were determined through electronic circular dichroism (ECD) calculations. The protective activity of the isolated compounds against corticosterone (CORT)-induced damage in PC12 cells was assayed, and 2, 4, and 11 exhibited definite neuroprotective effects with survival rates of 82.14 %, 72.63 %, and 74.36 %, respectively. Flow cytometry and western blotting analyses indicated that the neuroprotective effect of 2 might be attributed to the alleviation of CORT-induced mitochondrial-dependent apoptosis.
