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Platelet collection using the IBM 2997 cell separator.

R Bond, L Wood, P Jacobs

    Journal of Clinical Apheresis
    |January 1, 1985
    PubMed
    Summary
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    This study demonstrates that the IBM 2997 Blood Fraction Separator effectively harvests therapeutic platelets. Harvested platelets function normally in vivo and in vitro, with only mild, correctable donor hypocalcemia observed.

    Area of Science:

    • Hematology
    • Transfusion Medicine
    • Blood Component Collection

    Background:

    • Therapeutic platelet collection is crucial for managing thrombocytopenia.
    • The IBM 2997 Blood Fraction Separator is a device used for apheresis procedures.
    • Assessing the efficacy and safety of platelet harvesting techniques is essential.

    Purpose of the Study:

    • To evaluate the efficacy and safety of platelet harvesting using the dual-channel module of the IBM 2997 Blood Fraction Separator.
    • To assess the in vivo and in vitro function of harvested platelets.
    • To identify any adverse reactions in donors and recipients.

    Main Methods:

    • Performed 320 plateletpheresis procedures using the IBM 2997.
    • Infused harvested platelets into recipients with hypomegakaryocytic thrombocytopenia.

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  • Conducted additional plateletphereses in seven volunteers for functional studies.
  • Utilized radiochromium labeling for in vivo recovery and lifespan assessment.
  • Performed in vitro tests for platelet factor III availability and aggregation.

    • Main Results:

    • Collected 5.1 +/- 1.5 X 10(11) platelets per procedure.
    • Achieved significant platelet count increments in recipients.
    • Observed mild, correctable hypocalcemia in donors as the only consistent reaction.
    • Donor platelet counts decreased significantly (p < 0.01) post-procedure.
    • Harvested platelets showed normal in vivo recovery (59.4 +/- 3.4%) and lifespan (218 +/- 12 hr).
    • In vitro functional tests and ultrastructural appearance of harvested platelets were normal.

    Conclusions:

    • The IBM 2997 Blood Fraction Separator is effective for therapeutic platelet collection.
    • Harvested platelets retain normal function, making them suitable for transfusion.
    • The procedure is safe for donors, with manageable side effects.