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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Transmission Electron Microscopy01:15

Transmission Electron Microscopy

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Cryo-electron Microscopy01:28

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Related Experiment Video

Updated: May 4, 2026

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
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Electron microscopy as a tool for scientific illustrators.

M J Mann, E R Hodges

    The Journal of Biocommunication
    |May 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) provide detailed micrographs. These images aid medical and biological illustrators in accurately depicting small specimens with high-resolution surface detail.

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    Area of Science:

    • Microscopy
    • Scientific Illustration
    • Biological Imaging

    Background:

    • Medical and biological illustrators require high-resolution references for depicting small specimens.
    • Micrographs from electron microscopy offer unparalleled surface detail and clarity.

    Purpose of the Study:

    • To explain the functional principles of scanning electron microscopes (SEM) and transmission electron microscopes (TEM).
    • To present techniques for applying electron microscopy images in scientific illustration.

    Main Methods:

    • Review of the fundamental operational principles of SEM and TEM.
    • Demonstration of micrograph interpretation for anatomical and surface feature accuracy.

    Main Results:

    • Electron microscopy provides exquisite, high-resolution surface and internal details of biological specimens.
    • Micrographs serve as invaluable visual references for accurate scientific illustrations.

    Conclusions:

    • SEM and TEM are powerful tools that significantly enhance the accuracy and detail achievable in medical and biological illustrations.
    • Understanding electron microscopy techniques empowers illustrators to leverage these advanced imaging methods effectively.