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Published on: January 23, 2015
Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells for the Study of Macular Degeneration
Almar Neiteler1, Shyamanga Borooah2, James A Ross1
1Tissue Injury and Repair Group, Chancellor's Building, University of Edinburgh, Edinburgh, UK.
Abstract:
We have developed a method to reliably generate retinal pigment epithelium (RPE) in bulk from human induced pluripotent stem cells (iPSCs). These iPSC-RPE showed a typical RPE-like morphology with pigmentation and expressed typical RPE markers. The iPSC-RPE also show a transepithelial electrical resistance and can phagocytose photoreceptor outer segments, indicating that they are also functional. Large quantities of iPSC-RPE can be generated with this method through serial expansion for disease modeling and high-throughput screenings.
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