Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody-drug conjugate-related marker heterogeneity between primary tumors and metastatic lymph nodes in advanced urothelial cancers.

Journal of translational internal medicine·2026
Same author

Sleep duration and depressive symptoms among older Chinese adults: a serial mediation model of self-rated health and frailty.

BMC geriatrics·2026
Same author

Bidirectional longitudinal associations between social activity and depressive symptoms among Chinese older adults with multimorbidity: the moderating role of subjective life expectancy.

BMC psychiatry·2026
Same author

The global economic burden of diabetes for 190 countries 2021-50: a macroeconomic modelling study.

Nature communications·2026
Same author

Thematic characteristics and research hot-spots of China's healthy aging policy: topic modeling study.

BMC health services research·2026
Same author

The Relationship Between Emotional Eating Behavior and Internet Addiction in Junior High School Students: A Cross-Sectional Study.

Nutrients·2026

Related Experiment Video

Updated: May 14, 2025

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
14:04

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

15.2K

Neutrophil-to-lymphocyte ratio predicts a poor prognosis for penile cancer with an immunosuppressive tumor

Xingliang Tan1,2,3, Yanjun Wang1,2,3, Yiqi Yu4

  • 1Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Frontiers in Immunology
|May 1, 2025
PubMed
Summary
This summary is machine-generated.

The neutrophil-to-lymphocyte ratio (NLR) is a significant independent prognostic indicator for penile cancer (PC). A high NLR predicts poor outcomes and an immunosuppressive tumor microenvironment in PC patients.

Keywords:
cancerneutrophil extracellular trapsneutrophil-to-lymphocyte ratiopropensity score matchingtumor-associated neutrophils

More Related Videos

Isolation and Characterization of Neutrophils with Anti-Tumor Properties
10:15

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

24.7K
Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis
07:32

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Published on: April 12, 2024

1.1K

Related Experiment Videos

Last Updated: May 14, 2025

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
14:04

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

15.2K
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
10:15

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

24.7K
Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis
07:32

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Published on: April 12, 2024

1.1K

Area of Science:

  • Oncology
  • Immunology
  • Inflammation Research

Background:

  • Penile cancer (PC) is often triggered by chronic inflammation linked to poor genital hygiene.
  • The neutrophil-to-lymphocyte ratio (NLR) is a recognized marker of systemic inflammation with potential prognostic value.
  • Previous research on NLR in PC was limited by small sample sizes and confounding factors.

Purpose of the Study:

  • To investigate the prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in penile cancer (PC).
  • To assess the association between NLR and the tumor microenvironment (TME) in PC patients.

Main Methods:

  • Analysis of a large multicenter cohort of 582 PC patients undergoing radical inguinal lymphadenectomy.
  • Utilized univariate and multivariate Cox regression and propensity score matching (PSM) for prognostic analysis.
  • Employed immunofluorescence to evaluate the immunosuppressive TME and neutrophil infiltration.

Main Results:

  • High preoperative NLR (≥ 3.0) correlated with advanced tumor stage, lymph node metastasis, and lymphovascular invasion.
  • NLR and pN stage were identified as independent prognostic indicators for PC post-PSM.
  • Elevated NLR was linked to shorter progression-free survival (PFS) and reduced response to chemotherapy and immunotherapy.
  • NLR associated with increased proinflammatory cytokines, N2 tumor-associated neutrophils (TANs), and CD8+ T-cell exhaustion.

Conclusions:

  • The neutrophil-to-lymphocyte ratio (NLR) serves as a simple, effective, and independent prognostic marker for penile cancer.
  • A high NLR indicates an immunosuppressive tumor microenvironment and predicts unfavorable outcomes in PC.
  • NLR-associated N2 TANs and neutrophil extracellular traps may drive tumor progression and treatment resistance.