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Identification of the gene signatures related to NK/T cell communication to evaluate the tumor microenvironment and prognostic outcomes of patients with prostate adenocarcinoma

  • 0Department of Urology, Affiliated Hospital of Nantong University, Nantong, China.
Frontiers in Immunology +

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May 1, 2025

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May 1, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identifies key communication genes in prostate adenocarcinoma (PRAD) by analyzing NK/T cell interactions. These findings highlight potential new therapeutic targets for PRAD, a major cause of male mortality.

Area Of Science

  • Oncology
  • Immunology
  • Bioinformatics

Background

  • Prostate adenocarcinoma (PRAD) is a significant cause of male mortality.
  • Natural Killer/T (NK/T) cell communication is a critical research area in PRAD.

Purpose Of The Study

  • To identify key communication genes and cell-cell interactions in PRAD.
  • To develop a risk model for stratifying PRAD patients.
  • To explore potential therapeutic targets for PRAD.

Main Methods

  • Single-cell RNA sequencing data analysis using Seurat and CellMarker 2.0.
  • Construction of cell-cell interaction networks with CellChat.
  • Biomarker identification and risk model development using Cox and LASSO regression.
  • Immune infiltration analysis (ssGSEA, TIMER, ESTIMATE) and pathway analysis (GSEA).
  • Validation of gene effects on PRAD cells through in vitro assays.

Main Results

  • NK/T cells showed significant alterations and high communication intensity in PRAD tumors.
  • A risk score model was developed, differentiating high-risk and low-risk patient groups.
  • High-risk group exhibited increased immune infiltration and immune escape, while the low-risk group showed enrichment in metabolism-related pathways.
  • Seven biomarkers were validated in PRAD, with ISYNA1 knockdown inhibiting cancer cell proliferation and metastasis.

Conclusions

  • This research uncovers crucial communication genes within PRAD.
  • Identified genes offer novel therapeutic targets for PRAD treatment.
  • The study provides insights into immune cell communication in prostate cancer progression.

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