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Updated: May 9, 2025

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Electrophysiological analysis of cardiac KATP channel
1Cyrus Tang Medical Institute, Soochow University, Suzhou 215123, Jiangsu, China.
Abstract:
ATP-sensitive potassium (KATP) channels are integral components in excitable cells, particularly in cardiomyocytes, serving as critical regulators of cellular metabolism and electrical excitability. In instances of prolonged oxygen deprivation or heightened metabolic requirements, the opening of KATP channels enables potassium efflux by virtue of a diminished ATP/ADP ratio. This process aids in maintaining membrane potential stability, thereby mitigating excessive excitability and cellular contraction, ultimately contributing significantly to cardiac protection. The accurate isolation of intact single cardiomyocytes and the electrophysiological evaluation of KATP channels are pivotal processes in research on KATP channels in cardiomyocytes in vitro. Here, we present a comprehensive protocol not only for the efficient isolation of viable cardiomyocytes from the adult mouse through the Langendorff perfusion method, but also for the recording of KATP channel currents in single cardiomyocytes employing patch clamp technique.
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