Sensitive liquid chromatographic method for physostigmine in biological fluids using dual-electrode electrochemical...

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Physostigmine is a crucial drug with therapeutic applications.
• Accurate quantification of physostigmine in biological matrices is essential for pharmacokinetic and pharmacodynamic studies.

Purpose of the Study:

• To develop and validate a sensitive liquid chromatographic method for physostigmine determination.
• To establish a method suitable for high-throughput analysis in biological fluids.

Main Methods:

• Development of a liquid chromatography method utilizing dual-electrode detection.
• Implementation of a single solvent extraction step for sample preparation.
• Utilization of autoinjection for enhanced sample throughput.

Main Results:

• The developed method achieves a limit of detection in the range of 25-50 pg/mL of plasma.
• The method demonstrates high sample throughput due to efficient extraction and autoinjection.
• Data from oral physostigmine doses were successfully acquired and analyzed.

Conclusions:

• The dual-electrode detection liquid chromatography method provides a sensitive and efficient means for physostigmine quantification.
• This method is suitable for analyzing physostigmine in biological fluids, supporting clinical and research applications.