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A High-Throughput Platform for Culture and 3D Imaging of Organoids
Published on: October 14, 2022
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Human Organoids as an Emerging Tool for Genome Screenings
Francesco Andreatta1, Delilah Hendriks1, Benedetta Artegiani1
1Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands; email: d.f.g.hendriks-7@prinsesmaximacentrum.nl, b.a.artegiani@prinsesmaximacentrum.nl.
Annual Review of Biomedical Engineering
|May 1, 2025
Summary
CRISPR-Cas screenings in organoids are revolutionizing human biology research. This review explores strategies, challenges, and findings from these powerful gene editing tools in organoid models.
Area of Science:
- Biotechnology
- Genomics
- Developmental Biology
Background:
- Organoid models recapitulate human development, disease, and tissue repair.
- Organoids are derived from tissue stem cells or pluripotent stem cells (PSCs).
- CRISPR-Cas gene editing has advanced organoid research.
Purpose of the Study:
- To review the adaptation of CRISPR-based screenings in organoids.
- To examine strategies, scopes, and readouts of CRISPR screenings in organoids.
- To highlight challenges and discuss findings from organoid genome screening studies.
Main Methods:
- Review of recent literature on CRISPR screenings in organoids.
- Analysis of strategies for performing CRISPR screenings (e.g., scopes, readouts).
- Discussion of challenges specific to organoid platforms.
Main Results:
- CRISPR screenings in organoids have uncovered novel genes in various biological processes.
- The review examines different approaches and outcomes of these screenings.
- Organoid-specific challenges in implementing CRISPR screenings are discussed.
Conclusions:
- CRISPR screenings in organoids are a powerful tool for understanding human biology.
- Widespread adoption can significantly advance the field.
- Future outlook focuses on leveraging these techniques for deeper biological insights.
Keywords:
CRISPR-Casgenetic engineeringgenome screeninghuman organoidspluripotent stem cellstissue-derived organoidsMore Related Videos
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