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Related Experiment Video

Updated: May 9, 2025

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
09:33

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

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Engineered micro-structured biomimetic material for modelling the outer blood-retinal barrier.

Chloé Dujardin1, Walter Habeler2, Paola Aprile1

  • 1Université Paris Cité, Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science (LVTS), INSERM U1148, 75018, Paris, France.

Biomaterials
|May 1, 2025
PubMed
Summary

Researchers developed a novel scaffold to model the outer blood-retinal barrier (oBRB), crucial for understanding retinal diseases like macular degeneration. This biomaterial supports physiological cell organization for improved disease modeling and therapeutic development.

Keywords:
Freeze-dryingIn vitro modellingOuter blood-retinal barrierPolysaccharide hydrogelPre-vascularizationTissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Retinal Tissue Engineering
  • Ocular Pathophysiology

Background:

  • The outer blood-retinal barrier (oBRB) is vital for retinal health and is compromised in major eye diseases, including age-related macular degeneration.
  • Current in vitro models of the oBRB often lack physiological relevance due to 2D cultures or non-physiological dimensions.
  • Degeneration of the retinal pigment epithelium (RPE) and choroid leads to photoreceptor loss and blindness.

Purpose of the Study:

  • To develop an innovative scaffold-driven approach for modeling the outer blood-retinal barrier (oBRB).
  • To create a physiologically relevant in vitro model that mimics the oBRB's structure and dimensions.
  • To provide a platform for studying retinal pathologies and exploring therapeutic strategies.

Main Methods:

  • Engineered a polysaccharide membrane using freeze-drying to create a scaffold mimicking the oBRB structure.
  • Developed a single-piece functional material with a non-porous surface for RPE monolayer culture and an internal 3D porous structure for choroidal network formation.
  • Utilized hiPSC-derived RPE and co-cultured endothelial cells and pericytes within the scaffold.

Main Results:

  • The engineered scaffold successfully mimicked the oBRB's physiological dimensions and structural organization.
  • The 3D porous inner structure facilitated the self-organization of endothelial cells and pericytes, forming a functional choroidal network.
  • The material allowed close proximity and interaction between RPE and choroidal compartments while maintaining their distinct locations.
  • The scaffold demonstrated cyto-compatibility, ease of use, and cost-effective large-scale production.

Conclusions:

  • The novel scaffold-driven approach provides a physiologically relevant biomaterial for modeling the outer blood-retinal barrier.
  • This innovative model system offers a valuable tool for advancing the understanding of retinal diseases and developing new therapies.
  • The off-the-shelf, cost-effective nature of the membrane facilitates broader research applications in retinal tissue engineering.