Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 9, 2025

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

Optimizing intracameral injection for targeted gene therapy and glaucoma model development.

Shijiu Chen¹, Lin Cong², Xinlei Zhu²

¹Qingdao University, Qingdao, China; Eye Institute of Shandong First Medical University, Eye Hospital of Shandong First Medical University (Shandong Eye Hospital), Jinan, China; State Key Laboratory Cultivation Base, Shandong Key Laboratory of Eye Diseases, Qingdao, China; School of Ophthalmology, Shandong First Medical University, Jinan, China.

Experimental Eye Research

|May 1, 2025

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting the APOE4-driven peripheral-central immune axis: A new frontier for Alzheimer's disease therapy.

Pharmacological research·2026

Same author

Trends, survival and regional control of sentinel lymph node biopsy versus axillary dissection in cN0 breast cancer: a multicenter cohort in China.

Frontiers in oncology·2026

Same author

Optimizing Postoperative Management in Deficient Mismatch Repair/Microsatellite Instability-High Gastric or Gastroesophageal Junction Adenocarcinoma: A Multicenter Retrospective Study.

Journal of gastric cancer·2026

Same author

Multimorbidity burden and patterns associated with DeepBrainNet-derived brain-age gap in dementia-free older adults: A community-based study.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Same author

The association between brain oscillatory activity and immediate memory under different magnetoencephalography paradigms: A population-based study.

NeuroImage·2026

Same author

Curculigoside Alleviates CSDS-induced Depressive-like Behavior by Modulating Pyramidal Neuron Excitability and Synaptic Transmission.

Neuromolecular medicine·2026

This study introduces a simplified intracameral injection technique for mice, improving anterior chamber access for research. The novel method reduces complications and tissue damage in ocular animal models.

Area of Science:

Ophthalmology
Veterinary Medicine
Surgical Techniques

Background:

Intracameral injection is vital in animal research for glaucoma models, gene therapy (AAV), and drug delivery.
Performing intracameral injections in mice is challenging due to small eyes and shallow anterior chambers.
Traditional methods using air or viscous agents cause leakage, require repeat injections, and risk corneal injury.

Purpose of the Study:

To develop a simplified and safer intracameral injection technique for mice.
To minimize tissue damage and complications associated with ocular injections in rodents.
To provide a reproducible method for delivering substances to the anterior chamber in mice.

Main Methods:

A novel technique involving posterior chamber needle entry before accessing the anterior chamber through the pupil.

Keywords:

AAV transduction Corneal endothelium Glaucoma model Intracameral injection

More Related Videos

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Published on: May 31, 2024

Intravitreal Injections in the Ovine Eye

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

Related Experiment Videos

Last Updated: May 9, 2025

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Published on: May 31, 2024

Intravitreal Injections in the Ovine Eye

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

Validation using adeno-associated virus (AAV) delivery for corneal endothelial cell transduction.

Application in establishing a glaucoma model via magnetic microbead injection.

Main Results:

The simplified technique effectively delivered AAV for corneal transduction.
Magnetic microbeads were successfully injected to create a glaucoma model.
The method demonstrated efficacy, reproducibility, and reduced complications compared to traditional approaches.

Conclusions:

The novel intracameral injection technique offers a simplified, safer, and more effective approach for mouse ocular research.
This method minimizes tissue damage and complications, enhancing the reliability of experimental outcomes.
It provides a valuable tool for advancing research in ophthalmology and related fields using rodent models.